1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Supersedes 1. SoftMax® Pro 7. The adapter is required for optimum performa nce with standard 96-well and 384-. 425 or. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Page 28 Chapter 5: Read Modes and Read Types 1. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 7°C and the bottom to 36. Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. 1700 Online: Sales Offices USA/Canada 800. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. W×D×H. Testing can be done in the user’s lab on their own schedule. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Microplate Reader, Multimode Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 1-1. Reader Documentation Version No. The plate reader. coli stock with an OD 600 value of 0. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. ” So, acquisition and analysis come from one software system. 2. Temperature control and shaking come standard. Microplate Stacker and Washer. The reader measures both flat and round 96-well microplates and. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. HTRF-compatible SpectraMax Multi-Mode Readers. EMax. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Turn on Plate Reader and wait for it to complete its start-up routine. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. With SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Learn how you can utilize microplate readers to drive landmark discoveries. The Versamax Microplate Reader is built around a grating monochromator. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). ** Some compatible filter sets are available for purchase from Life. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . 0200-6263. 5577 UK +44. 944. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. No accessories are needed and SoftMax® Pro Software. SpectraMax Microplate Readers. One plate carrier adapter is provided with the instrument. ] Example of document to evaluate if the reader . Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Select Start>Control panel>Device Manager. The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. Open the top cover of the instrument. 2. Category 2 and 3 microplate readers use gradient heating as well. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Products : General, FilterMax F5 reader. 9. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 1. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for . If it looks fine, then proceed. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. 1. Increase productivity with patented microplate reader technologies. Integrated to. The adapter is required for optimum performance with standard 96-well and 384-. We would like to show you a description here but the site won’t allow us. It is more inert and reduces compound interference. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. HTRF-compatible SpectraMax Multi-Mode Readers. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. #4TI-0655) Microplates. The lower limit of detection (LLD) was defined as the level of. Superior opticsThe top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. That’s why Molecular Devices offers the following services: Installation qualification (IQ), operational qualification (OQ), preventive maintenance (PM), and repair coverage. Figure 10: The Plate section Template Editor, showing the series set up. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Measure more accurately. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Fluorescence Microplate Reader Comparison Chart. 1. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. These lab workhorses are used in drug discovery. Step 3: Click “Settings” and a screen will appear (Figure 1). begin reading microplates with seven mouse clicks. Microplate Stacker and Washer. We would like to show you a description here but the site won’t allow us. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. SoftMax Pro’s 21 curve fit options, including 4-parameter logistic and 5-parameter logistic models, and the ability to assign multiple standard curves within the same plate make it easy to interpolate unknown samples or perform parallel line analysis (PLA) to determine relative potency. This technical note is designed to provide information . Plate stacker and robot integration The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within minutes and begingene expression with a user-friendly reader. Read height should be optimized forIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Details. 0 . SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. Monday to Friday 8am - 5pm GMT. Use the 1. Image courtesy of Molecular Devices. In . 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Uncontrolled Copy for Training and Reference Purposes Only . Microplate Readers. ABS Test results. White plates reflect the light, enabling even low levels of Luminescence to be detected. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The plate reader optics are. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Fig 2: Enzymatic response of synthetic compounds. The SpectraTest® ABS1 Absorbance Validation Plate**4 °C above ambient to 45 °C. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. Get the most out of SoftMax Pro. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Figure 1. Eliminate time-dependent substrate addition steps. How to import latest protocol revisions into SoftMax Pro Protocol Library; Protocols for the ABS2 Validation Plate. Molecular Devices' microplate readers. Detection cartridge modularity allows for easy addition of new read modes within minutes without. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. #SpectraMax L Config) Methods. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. The computer controlling the plate reader runs Windows XP. 1. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The plate was incubated at room temperature for 50 minutes or 2. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 0 OD for earlier models. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Dual monochromators increase performance and assay flexibility. SpectraMax® Microplate Readers and SoftMax® Pro Software. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor Jinfang Liao and Evelyn McGown Molecular Devices Corporation, 1311 Orleans Dr. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Experimental details and customization of the group table. Absorbance Plate Reader Comparison Chart. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software Check software compatibility with Windows 10 and locate your software installer. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. We would like to show you a description here but the site won’t allow us. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 2. S. Find regional support. in steps of 0. Flexibility to read a variety of plate formats. Exploring misconceptions and understanding the technologies. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The advantage of using labeled probes is detection specificity. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. com #THX1138 Introduction The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes. Two. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Molecular Devices' microplate readers. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. In the M2, M5 and Plus. Cell viability는 세포막 Integrity 또는. 1-10 sec. Access all the resources, tools and information you need to stay productive. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Cell line development teams will realize savings on time and resources,SpectraMax i3x. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 313 reference gain check fail. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Note that this procedure is only for the SpectraMax L model. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Also refer to the Multimode Reader Comparison Chart Features. We would like to show you a description here but the site won’t allow us. Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Vmax® plate reader specifications. Molecular Devices training courses and tutorials. SpectraMax ABS and ABS Plus Microplate Readers are a compact and tunable absorbance microplate readers. Cell Cytotoxicity assay procedureMolecular Devices. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535. The low profile increases robotic stack capacity and storage. 211, and 820 and EudraLex Volume 4 and Annex 11 and 15, and how they apply to the implementation of Molecular Devices microplate readers and SoftMax Pro. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. In this unique new five-part weekly webinar series, running every Thursday throughout October 2020, we’ll introduce you to various aspects of microplate reader technology in an educational and entertaining way! Starting with some basic concepts,. System setup and calibration are controlled from within SoftMax Pro Software. Turn the reader off by pulling the power cord. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Multimode Microplate Readers. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:[The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Plate section without PathCheck applied. When the user applies a set temperature of 37°C, the top is heated to 37. Label-free detection. ®This document is provided to customers who have purchased Molecular Devices, Inc. Double-click the unknown device with the yellow warning icon. (b) Fluorescence responses of probe HADP and control compounds. ®Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. High-precision 9 channel system; filter-less with up to 6 wavelengths. Shaking. Published Dec 16, 2020 | Updated Nov 08, 2023. A kinetic plate read was initiated at time zero. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. This multi plate reader is excellent to work with for checking the U. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. The instrument supports the following read modes:. Gregory et al. ISM Equipment Classification (Group 1, Class A) This equipment is. The system provides automation for up to 50 microplates for easy walkaway automation. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12Plate reader instructions Computer. Assay flexibility. Molecular Devices also offers Digital IQ/OQ/PM. Avoid losing high signal. Description Figure 1. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. FLIPR Calcium 4 Assay Kit. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. SpectraMax Microplate Readers. Step 2: Launch SoftMax Pro Software. Our extensive SpectraMax® line of user-friendly microplate readers – the. If the computer is connected to the Internet: Select Search Automatically for updated driver software. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 25 to 3. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Important things to consider when purchasing a multimode microplate reader. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 1. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. This offer includes SpectraMax i3x, SpectraMax. Supplier: Molecular Devices. 18 kg. Imaging System Giles Scientific Biomic V3 Automatic Plate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 0 . e. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 0. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. FlexStation 3 Multimode Microplate Reader (Molecular Devices Cat. #655096) • Brown or amber (light-blocking microcentrifuge tubes • Molecular Devices microplate reader with fluorescence detection mode:What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. An industry-renowned instrument for monitoring GPCRs and ion channels. SoftMax Pro. Complete plate reader solution for all your research needs. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. Every step is optimized to simplify analysis and reporting to support our microplate readers. 303 unable to cal dark current. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The drawing shows a standard microplate of 15 mm height covered with a lid. Find our technical support information along with contact details. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 3 foot) See. • Analyze data in a single plate, across multiple plates, or between different groups. 1. 9. 8 foot) 4400-0002 Power cord, 1 meter (3. The drawing shows a standard microplate of 15 mm height covered with a lid. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is preferred for luminescence rather than the core’s multimode readers. For nearly 40 years, we have partnered with scientists to help them achieve landmark discoveries. Contact Us Request Quote Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Superior optical system includes a xenon flash lamp and features. Important things to consider when purchasing a multimode microplate reader. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. FlexStation™ II Systems provide integrated fluid transfer capability from a source platePlate Reader SpectraMax iD5 multi-mode system Plate Washer AquaMax 4000 Microplate Washer Robot PreciseFlex 400 Sample Handler Plate Storage Plate Hotels – 40 microtiter plates Controlling Software Genera Control Software Incubation LiCONiC STX44 Automated CO 2 Incubator Liquid Handler Microlab STAR Automated Liquid Handling The. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 304 signal level saturation. The adapter is required for optimum performa nce with standard 96-well and 384-. By measuring optical pathlength, it eliminates the last. Unparalleled performance on a personalized platform. Figure 9. 5 µL of each sample was pipetted into a 96-well. Select Update Driver. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The Back Panel Figure 1. 1 sec. Create custom fluorescence assays without having to purchase expensive filter sets. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. 1. PURPOSE. After reading the ELISA plate on both readers, each standard curve was nearly identical. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Plate Formats: 6 to 384-well microplates. \r ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Toggle navigation 0 Cart; Store. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. (a) Chemical structures of 1 (HADP) and its analogues 2–4. in steps of 0. When connecting for the first time to either the SpectraMax L reader or StakMax system. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Fluorescence Microplate Reader Comparison Chart. 5 sec. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. Viewing results on your new SpectraMax iD3 Multi-Mode Microplate Reader. First, as mentioned before, the walkaway time significantly. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Step 3. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. Molecular Devices Software. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 2. The whole plate can be detect in a single run with ease and flexible. ensure your reader is thoroughly tested against specifications we consider critical to achieving quality results. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Microplate Reader Shelf. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 7°C (gradient heating design). Citations on Google Scholar. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. They feature options and modules to address your specific research including objectives, filters, imaging modes, and environmental. The plate reader can measure multiple readouts, including UV and. The plate. 5: Schematic of the back panel of a reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. book Page 1 Tuesday, July 6, 2010 2:08 PM SpectraMax Microplate Readers. 9. Plate pre-read measurements were made in a Costar UV plate. Endpoint. a single plate read using any standard cuvette, or 96- or 384-well microplate. San Jose, California, June 5, 2018. Turn on Plate Reader and wait for it to complete its start-up routine. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. A control well was set up to verify the accuracy of the standard curve. Complete plate reader solution for all your research needs. Some familiarity with Windows should be sufficient to use it. 10,700. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Please click on the links below to view the Microplate Reader Comparison Tables. Luminescence reading is a secondary mode of the Gemini instruments. Spectranet customer portal. Clone Screening. 2 Software Release Notes. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. With optimized reagents, option to operate the. Multimode microplate readers offer flexibility, and ease of use. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Monday to Friday 7am - 5pm Pacific Time. If the computer is connected to the Internet: Select Search Automatically for. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. Select the Driver tab.