a white 96-well half-area microplate (Corning cat. 1 fmol luciferase per well. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. Polypropylene deep well plates, round bottom. SpectraMax i3x Multi-Mode Microplate Reader. #E010) Pyroplate 96-well microplate (ACC cat. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The CellTiter-Glo assay was used to measure ATP. No tools required to assemble. 25% and 0. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. It also integrates with the powerful SoftMax Pro Software for. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. the Molecular Devices SPECTRAmax® 190 and 250. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. This reader also makes use of a unique temperature-independent methodology for path length correction. An entire 96-well plate can be processed in less than six seconds. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. validation, maintenance, and repair documentation. Plus 384. UV Plate 96 Well, Flat Bottom, Cs 50. x. Endpoint, kinetic, spectrum. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Silica excitation fiber, glass emission fiber. UV absorbance detection. 6-384 (96- and 384-well with pipetting) 96-well microplates. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Fluorescence Microplate Reader Comparison Chart. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Molecular Devices' microplate readers. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 96-well plates - Filter plates with 0. 0% ± 0. 4 (Molecular Devices cat. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). SpectraMax iD3 reader with touchscreen and inserted USB flash drive. , Corning, NY) or Greiner. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Back plate and…. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Selects either 96-well or 384-well plate for a stand-alone read. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). 057, depending on lot number. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Temperature control range from Ambient +4º to 50ºC. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Cell viability and cytoxicity assays. Fluorescence Microplate Reader Comparison Chart. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. SpectraDrop High-Throughput Screening (HTS) Kit. UV Plate 96 Well, Flat Bottom, Cs 50. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. 305 reference level saturation. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. x. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 314 low lamp level warning. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. They can be read by most microplate readers. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. No accessories are needed and SoftMax® Pro Software. M2. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. It features a modular design that allows users to add or change detection cartridges according to their needs. FAST AND ACCURATE METERTECH Microplate Reader $3,650. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Order Now. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Tristar 3 Multimode Microplate Reader. ₹ 1,80,000 Get Latest Price. to each well. Detection of KASP genotyping reactions. 2. The system features 96, 384,. 7°C and the bottom to 36. EMax. x. Page 1 of 8 . SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 000 OD ± 1. Anticancer drug assay was also conducted using conventional 96-well plates as controls. FlexIII. 01. Loading Buffer was also added to a set of cell-free wells for background controls. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. Some will only support one well density (e. Dynamic range > 6 decades . 005 OD Normal and Rapid modes, 96- and 384-well plates;. U-bottom: 30-40 µl. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. 312 gain calibration failed. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Expand Microplates and select the newly created plate. . Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. 5. S. Categories: Plate Readers. . Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. 2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Get Quote . x. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 0 . Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. x. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Spectrofluorometer from Molecular Devices® provides a flexible environment. C. 9. 96-, 384-, and 1536-well microplates. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Part of a plate can be selected so that only a selected area of the plate. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 5 ng per well. 0. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Supersedes 1. Time-resolved phosphorescence. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Incubation. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Usage/Application: Lab. 2. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The VersaMax reads 96-well microplates. Three low volume 24-well bottom slides. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Microplates, cuvettes (via adapter) Detectors. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Running both standard spectrophotometer and microplate. • 0. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). 313 reference gain check fail. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. A = Log 10 (I 0 /I). Compare specifications and request a quote from leading suppliers. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. Users can choose to process all plates within the magazine or select a specific number. Published Dec 16, 2020 | Updated Dec 16,. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. x. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. perfect for kinetic readings involving growth curves at 5 seconds per plate. x. High-throughput, automated dynamic light scattering. . The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. White 96-well platePlate formats. 3% in white 96- and 384. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Corning. Z. Next-generation luminescence plate reader. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Contents of wells were pipetted up and down several times to mix. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. No. Gemini EM Reader scans. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. Sample by eye within . This can increase throughput and efficiency, thus saving time and space in the lab. HeLa. VMax . S. Molecular Devices SpectraMax Plus 384 Absorbance. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. Our. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. No. Click Add to create the plate. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 20 attomol ATP per well. The color touch screen provides a visual user interface, making programming fast and intuitive. Now select the area of the plate to read. In SoftMax Pro, Dual Read mode was selected. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. BMG Labtech - THERMOstar. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. This page contains the. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Making it possible to perform kinetic analysis of solid. Instrument pricing. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Cayman Chemical. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Important things to consider when purchasing a multimode microplate reader. These microplates have a corner notch for use in standard plate readers. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. Optional 16-way manifold. When the user applies a set temperature of 37°C, the top is heated to 37. 1. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. These lab workhorses are used in drug discovery. #iD5-STD). #ABS Plus) Cell plating and treatment prior to assay. Products : General, FilterMax F5 reader. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. 039. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. robotics-automation-compatible. Service Contracts, Preventative Maintenance and. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. When a. UV absorbance detection. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. All trademarks used herein are the property of. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 96- and 384-well. variety of. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Consequently, FLIPR has been used. g. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Answer. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. #3693; n = 4 for each standard). Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The picked. Molecular Devices. 1 x plates; Instrument dimensions. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Materials. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Absorbance cuvette port. Browse for different fluorescent readers below and compare across different instrument suppliers. 020 fmol 0. The VICTOR Nivo. Molecular Devices L-Max II Luminescence Microplate Reader. Reader Documentation Version No. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Lucid Scientific. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Supersedes 1. x. $14,995. $542. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. 304 signal level saturation. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. 1% in white 96- and < 0. x. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. #ABS PLUS) Methods. Compare this item. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Published Dec 16, 2020 | Updated Dec 16, 2020. Products : Ionworks HT, Ionworks Quattro. More Product Information. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. and glow luminescence modes in 96- to 384-well plates. *For Corning UV plates, mean values at 260 nm ranged from 0. 6. Category 2 and 3 microplate readers use gradient heating as well. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The luminescence was measured in a SpectraMax. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 00. A standard 96 well plate was set up as described in Protocol Table 4. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. We would like to show you a description here but the site won’t allow us. Temperature control: 4°C above ambient to 45°C. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Compact designs make these instruments. explore more. Multimode microplate reader equipped with latest detections like fluorescence,. Description. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Clear plates will give more cross talk between wells. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. #C1500) Control standard endotoxin (ACC cat. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Washers configured for both 96- and 384-well plates. 4 (Molecular Devices cat. $3,450. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. 000 to 2. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Cell viability and cytoxicity assays. Access this by going to. Figure 2. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. d. The. #23225). x. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The CellTiter-Glo assay was used to measure ATP. (Molecular Devices cat.