Standard curves were plotted using SoftMax® Pro Software (Figure 1). 1 g/ml SDS solution in 0. Enzyme activity from tissue homogenates was normalized to total protein content with the Bradford protein assay (Biorad). The assay was performed on HDL collected. SpectraMax Readers have the most complete level of product validation and compliance. Select the Driver tab. 2. Figure 9. The EC50 values were calculated from the absorbance values vs. One hundred microliters of the supernatant was then transferred into a 96-well plate and read in a SpectraMax M5 plate reader (Molecular Devices) at A570. 3. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. edu, 814-863-3503. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Fluorescence Microplate Reader Comparison Chart. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 2. 130 Watts max. both cuvette-based and microplate reader applications on the same instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. StakMax Handling Stacker Base Unit. Nonspecific binding was determined by competition with 1. Power tested, slide moves in and out of unit as. The standard PEG assay required larger volumes and thus higher amounts of protein (∼5X) compared to the micro. After 30 min, the plate was washed with PBS 0. 6. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Molecular Devices LLC temperature controlled molecular devices m5 plate reader Temperature Controlled Molecular Devices M5 Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Lucifer yellow concentrations in the donor and acceptor wells were measured in the last of this incubation. Turn the reader on and secure the USB cable. The absorbance at 450 nm was subsequently recorded with SpectraMax M5 plate reader (Molecular Devices). During the room temperature incubation (step 9. Release of free AMC by proteolytic activity was monitored on Molecular Devices M5 plate reader with excitation and emission wavelengths of 360 and 460 nm, respectively. Wide range of applications. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. Supernatant was diluted. The drawing shows a standard microplate of 15 mm height covered with a lid. The measured specific activity of NanoLuc is 1. d. 4. 1. We use Spectramax M5 microplate reader in our lab. Thank you!. Multiskan Verification Plate, 8. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Remove plate cover. The IC50 was calculated using the DRC package in the R software program (v3. 5: Schematic of the back panel of a reader. 1 and slit widths of 2. View the Molecular Devices website. Purified fluorescent protein was diluted 15-fold into 0. The emission of roGFP2 was measured between 500 and 530 nm after excitation at 405 nm and 488 nm every 30 s in a SpectraMax M5 plate reader (Molecular Devices). : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. BioTek 50TS Plate Washer; Chemidoc MP; C1000 Thermal Cycler; Fragment Analyzer 5200; Genesys 10S UV-Vis Spectrophotometer; Guava EasyCyte 5HT; KingFisher Flex; MinION; MiSeq; Nanodrop; QuantStudio 3D; QuantStudio 5; Qubit 4; Savant DNA 120 SpeedVac SpectraMax M5 Plate Reader; StepOnePlus qRT PCR; ST1R Centrifuge;. For a Price List, please call (858) 646-3100 ext. 13. . Please talk to your your local Tecan representative for the right configuration which suits your application demands. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. At predetermined time points, the CCK-8 reagent was added into representative wells and the plate was incubated at 37°C for 1 h. Page 8 SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide CAUTION! Use of organic solvents can cause harm to the optics in the instrument. xlsx file, with or without the averages and normalizations. The concentration of MMP-9 was measured using R&D System’s ELISA Kit (Minneapolis, MN) on a Spectramax M5 plate reader equipped with SoftmaxPro v5. Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. begin reading microplates with seven mouse clicks. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The sample is typically placed in a cuvette through which light is sent horizontally. In the closed conformation, the regulatory SH3 and SH2 domains pack against the back of the kinase domain, providing allosteric control of kinase activity. 09 in One Micro Manipulator Reader software. The cell lysate was then transferred to a white opaque 96-well plate (50 μl/well), mixed with the freshly diluted substrate (50 μl/well), and bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. To eliminate the differences of TCR expression levels among different JRT cell lines, relative luminescence unit (RLU) was normalized to the condition with Human T-Activator DynaBeads at 2:1 bead-to-cell ratio. HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. A 4-parameter fit using the SoftMax Pro software included with the M5 Plate Reader was drawn through the data and a correlation coefficient (R 2) value > 0. Most papers they cultured bacteria to OD600>0. Description. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Clear Filters. Extracting viral DNA from 4 x 109 copies of the AAV8 preparation ( nominally 5 ng ssDNA)The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Absorbance was measured at 563 nm using a SpectraMax M5 plate reader (Molecular Devices). SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Most papers they cultured bacteria to OD600>0. Information on how to reserve the plate reader Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Place the plate in the SpectraMax M5 plate reader immediately, read OD 405 nm every 30s, last 1 h, 37 °C. fluorescence). After the plate is read, data will appear in the plate section: 11. Most papers they cultured bacteria to OD600>0. 95. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. In my opinion one the best plate readers which I used till now is Molecular Devices SpectraMax M5 Plate reader. 5 mL, pH 7. Then, cells were treated with TCS of different concentra-tions (10, 20 and 40 μg/mL), according to theThe luminescent signal was quantified in a SpectraMax M5 plate reader (Molecular Devices, San Jose, CA). Models for In. Viability Assay reagent (Promega). coli cells were purchased from New England Biolabs (Ipswitch, MA). The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. 2. Duplicate wells were tested for each condition, and data were graphed as a percent of control in GraphPad Prism (GraphPad Software). 1. ELISAs were carried out according to the manufacturer’s instructions for IL-1β (BD Biosciences, San Diego, CA), TNFα, MIP-1α, and MCP-1 (R&D Systems, Minneapolis, MN) and quantified using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Description Figure 1. When the user applies a set temperature of 37°C, the top is heated to 37. 72 MB. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. Cell morphology HeLa & caski cells were, respectively, plated in 6-well plates to logarithmic growth phase. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Step 1: Open the SoftMax Pro IMAP FP protocol. For example, microplates containing different samp les can be read using the same orThe 526/470 ratios for the standards were plotted against the standard concentrations. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 303 unable to cal dark current. Turn on Plate Reader and wait for it to complete its start-up routine. Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. S. 9. 25 pM (0. Pretreatment serum was used to determine nonspecific. Plate chamber design of the SpectraMax category 1 microplate readers. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. 9000-0756. 11. 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 312 gain calibration failed. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with different modes and settings. The purple holder is used for reading non-transparent plates from the top (e. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. The EPOCH2 can easily develop various types of assays (wavelength scan: 200–999 nm), and its temperature control (up to 65 c) is excellent. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. ScanLater Western Blot Detection Cartridge. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 12. Assay for Misfolded SOD1. Readings were taken every 30 minutes. 1. Figure 11. 3. I’m. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. After the plate is read, data will appear in the plate. Table of Contents. After 2 h of incubation, the plates were shaken, and the absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm (OD450). Fluorescence measurements were performed at 30°C (S30 extract) or 37°C (PURE system) using a SpectraMax M5 Plate Reader (Molecular Devices) set to 610 nm excitation and 650 nm. Kojic acid was used as a positive control. The reduced readings obtained from the plate reader were analyzed using PRISM 6. Molecular Devices Spectramax M5 Microplate Reader; Related products. HSL_EQ_007: Use and Maintenance of a 2-8°C Refrigerator in the HPV Serology Laboratory 3. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. Use of Attune Flow Cytometer, Leica Cryostat, M5 Plate Reader, and Typhoon 5 require training; please contact Cell_Bio_Operations at hms. The Infinite and Spark multimode microplate readers are modular and upgradeable. For a higher degreeTime Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Primary human airway. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Microplate automation and detection instruments for walk away operation and high quality results. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The measured bioluminescence data were expressed as mean ± SE (n = 3) and fitted with one-site binding model using the. Cell–cell fusion assays were also performed by transient transfection of pHXB2-env plasmid [34] along with pRev and pTat plasmids into 293T cells and these were added to the receptor. Turn on Plate Reader and wait for it to complete its start-up routine. Cell apoptosis assay. Data reduction and calculation were performed with MSD Discovery workbench software (v 3. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: Step 4: Incubate at room temperature for 1 hour, protected from light. 5 hours after an initial 10-minute incubation period. 320 nm and em = 456 nm) on a Spectramax M5 plate. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB. Flock Safety is an Atlanta-based company that sells automatic license plate detection readers, or ALPRs for short, to law enforcement agencies, schools, neighborhood homeowners associations and. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Tablet PC. Description. 9000-0756. Abs). To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. 2. When the user applies a set temperature of 37°C, the top is heated to 37. With optimized reagents, option to operate the. When a feature or capability applies to only certain readers, this. Linear, Orbital Slow, and Orbital Fast. 3. 5 pM (0. reader (Molecular Devices). About. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SA-β-gal assay. 2. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 5. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. M5 plate reader at 595 nm. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The fluorescence was measured using a SpectraMax M5 plate reader at 358 nm excitation and 455 nm emission. BioTek Gen5 software is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning applications in a variety of microplates and other labware. Endothelial permeability was also measured in vitro, using a transwell assay. 315 can't find zero order. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. SpectraMax M5 Plate Reader: 901F: Nanodrop 2001: BioRad ChemiDoc gel documentation (old) Bio-Rad T100Tm Thermal Cycler : Bio-Rad CFX ConnectTM Real-Time System : Location. 2016 Aug 2; 7(31): 50027–50042. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. Wells with an OD 600[0. The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. Animal modelThe PEG precipitation assay was performed with VRC01-WT mAb at a final protein concentration of 0. 4. The Chemistry Department is housed in an air-conditioned building, sharing space with the Departments of Biology and Molecular Biology & Biochemistry. Add 100 μL of this. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. 0 mM of NADH per min at pH 8. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Plate Shaking. The absorbance at a wavelength of 540 nm was quantified using a Spectramax M5 plate-reader (Molecular Devices) to determine the amount of gamma glutamyl hydroxamate, indicative of active enzyme. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 5 and 10 nm were used for excitation and emission, respectively. SpectraTest ABS2 Absorbance Validation Package performance tests. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 1. Find regional support. Cytation 5 extends the legacy of Agilent BioTek multimode plate readers with a modular and upgradable imaging mode. Absorbance was measured at 405nm on a Molecular Devices SpectraMax M5 plate reader. Fluorescence experiments were carried out using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Information on how to reserve the plate readerMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 1. In general, reading fluorescence from the top is more sensitive than reading from the bottom. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Plate Analysis 9. Molecular Devices M5 plate readers; Multiple liquid bulk dispensers (Wellmate, Multidrop Combi) Price List. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Also refer to this Multimode Reader Comparison Chart. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Quick Guide to the plate reader. The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. This unit must survive. Fluorescein 0. After the plate is read, data will appear in the plate section: 10. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Plate Analysis 9. An emission wavelength of 535 nm was used for carboxy-H2DCFDA and 610 nm was used for DHE and MitoSOX ™. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. For more sample throughput, the system can be easily integrated into a full roboticCell–cell fusion levels were measured using a luciferase assay (One-glo; Promega) and measured on a Spectra Max M5 plate reader (Molecular Devices). The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Rating: 5. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. to meet all your research needs. After 24 h, cells were treated as described for the individual figures, then assays were performed 48 h after transfection. 3. Catalog number: 165306. 2. Reader Documentation Version No. With optimized reagents, option to operate the readers. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. ). Softmax Pro GxP Software 6. 2. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 2. In the M2, M5 and Plus. Published Dec 16, 2020 | Updated Dec 16, 2020. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. The plates were washed thrice with kit wash buffer; 150 µl kit substrate was then added to each well and the plate incubated 30 min at room temperature (with shaking). Typically the system can detect absorbance, luminescence, fluorescence,. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. The plate can be shaken (icon right of thermometer, and see automix option in setup). 0 and room temperature. This is a result of the light being attenuated and scattered by the. Reactions were performed on a 384-well plate, with release of 6-FAM dye monitored by fluorescence at 517 nm using a SpectraMax M5 plate reader (Molecular Devices) with excitation at 495 nm for 60. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. : 10 Mar 09 Page 1 of 14 Setup Guide on the BioTek Instruments FLx800 Fluorescence™ Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. -Glo assay solution (Promega) at room temperature and luminescence measured at 1000 ms −1 integration on a SpectroMax M5. 5: Schematic of the back panel of a reader. Microplate Reader Shelf. S6. 9. Three kits covering a wide range of dsDNA concentrations. 1 or 10 Tablet PC with USB connection to instrument. Briefly, Nunc microtiter plates were coated overnight at 2° to 8°C with KLH (3 μg/ml; IMMUCOTHEL, biosyn Arzneimittel GmbH) prepared in PBS. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Cells were then pulsed with 1μCi 3 H-thymidine (Perkin Elmer, Waltham. A SpectraMax M5 plate reader was able to continuously incubate the microtiter plate at 37°C and measure the fluorescence (λex/λex= 529/600 nm) every 5 minutes for 1. 10 μL of each SCGal-Ura and SC-Ura culture were then transferred to 1 mL fresh SC-URA and allowed to grow to saturation overnight. The absorbance was measured using a SpectraMax M5 plate reader from Molecular Divices (San Jose, CA, USA). The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. Simply unpack it, plug it in, and begin your experiments. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. g. Products. Simply unpack it, plug it in, and begin your experiments. Category 2 and 3 microplate readers use gradient heating as well. The concentration of infectious virus in both stock. Lysin A constructs were expressed in M. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. 316 grating motor driver faulty. 40266 Email: [email protected]) at room temperature. With SOFTmax PRO, the contents of the wells in a microplate can be mixedAdjusting fluorescence gain on microplate readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 4 software (Molecular Devices, San Jose, CA, USA). Appropriately. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. We would like to show you a description here but the site won’t allow us. 4. 306 plate air cal fail,low light. This user guide provides safety information, warnings, cautions, tips, and instructions for operating the instrument, connecting cables, setting up the instrument, and troubleshooting. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Chemistry Department Facilities. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. Highly selective for dsDNA over ssDNA or RNA. 5 pM (0. After that, the medium was. Protocol for Qubit DNA HS Assay. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode. The name is the same, but the attitude is completely different. The Back Panel Figure 1. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. 5 h (during the lag phase), 7 h (during the exponential phase), and 24 h (during the plateau phase) for analysis of the fermentation. The fluorescence measurements of cell experiments were conducted with a SpectraMax M5 plate reader. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Cells stably expressing wild-type or E633K p110β were. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Reader Documentation Version No. This procedure is illustrated in Fig. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. 9. 4: Test tube cover. , a positive control). Circular Dichroism. RoHS Compliant. 6 for further experiment, so I'm confused which i would choose for OD value determination. 0 . After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). CHO-K1 cells were transfected with luciferase. 9. Small molecule ligands that engage the regulatory. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. com 9. The plate reader. doi: 10. After 2 h of static incubation at room temperature, the OD 630 of the cell-free supernatants (A) and deionized water controls (Ar) was then measured using a plate reader (SpectraMax M5) at room. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Select the Driver tab. Documents. High tolerance for contaminants. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). Information on how to reserve the plate readerBioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing 50 nM ATP (sample). SpectraMax Injector Cartridge with SmartInject™ Technology. AC16 cells were treated with control, 5 mmol/L LiCl or 2. Reader Documentation Version No. 2 MΩ cm. HTRF-compatible SpectraMax Multi-Mode Readers. The amount of HRP encapsulated in liposome was calculated from the calibration curve of. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Human T-cells were cultured at 1 × 10 6 /mL for 5 days with plate-bound 10 μg/mL anti-CD3 and soluble 1 μg/mL anti-CD28.