Microplate Spectrophotometer. No accessories are needed and SoftMax® Pro Software. 48, 96, and 384 wells can be used in the SpectraMax M2 and SpectraMax M2e. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. #S1207. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. 0. 1 fmol luciferase per well. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Packed 25 plates per sleeve, 2 sleeves per case. Shaking: Linear, orbital, and double orbital. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. 1. the use of a reader calibrated with primary NIST and NMI standards. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. A ten-fold serial dilution of purified luciferase in PBS with 0. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 4 Equipment. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. x. , April 14, 2022 – Molecular Devices, LLC. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. 384-Deep Square Well Plates. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. 96-, 384-, and 1536. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. Pro, Dual Read mode was selected. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. EMax® Plus Microplate Reader, Molecular Devices Catalog Number: (10119-370) Supplier: Molecular Devices Description: Robust, high value microplate reader designed to. 더 많은. There is no product in the shopping cart, buy it!. Some have optional microplate readers, allowing emission scans in up to 384-well plates, for example. 2. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. Molecular Devices has provided scientists with tools to expand the boundaries of their research. Add to Cart. Published Dec 16, 2020 | Updated Dec 16, 2020. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. Microplate format. or use the modified protocol below for the generation of NPCs (see Note 1). Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. x. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Figure 1. ,LTD (CAT#: STEM-S. SpectraMax i3x. The washer has a small footprint and is vacuum and pressure free, The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. All stand-alone instrument functions can be controlled using the software. Absorbance cuvette port. Find regional support. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 384-Well Microarray/High Sample Recovery Plate. After the reaction was complete, 5 µL of each sample was transferred into wells of a 384-well plate and detected on the microplate readers using the instrument settings listed in Table 1. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 2. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. The assay methods covered include. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. com Check our website for a current listing of worldwide distributors. 2. Features. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 003 fmol 0. x. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Microseal 384-Well PCR Plates. #3-000-510)Corning® 384 well microplate, low volume poly-D-lysine coated surface, black polystyrene, round bottom, sterile, lid, pack of 10; Synonyms: 384 multiwell plates,384 well low volume plates,384 well microplates,384 well microtiter plates,384 well plates,384 well tissue culture treated plates; find -CLS3643 MSDS, related peer-reviewed papers,. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. the iD5 reader’s filter system. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Gemini EM Reader scans. 0 mP 96-well. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. EMax. #ABS Plus). Each kit is sufficient. +1 800. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. 20 attomol ATP per well. Step 1: Open the SoftMax Pro IMAP FP protocol. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Cross-talk <0. The FLEXstation II 384 (Molecular Devices, Inc. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. System setup and calibration are controlled from within SoftMax Pro Software. Application Area: Spectrophotometer for molecular biology. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. #N207) LAL Reagent Water (Lonza cat. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. By measuring optical pathlength, it. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Fluorescence Microplate Reader Comparison Chart. Select Start>Control panel>Device Manager. and the A1 corner of the plate adapter (Figure 1-2). 6-, 12-, 24-, 48-, 96-, and 384-well microplates; Extended research windows available; Temperature control; Returnable samples; Repeatable experiments;The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. x. Outer wells are aligned for proper viewing with FLIPR. Reliable performance with a proven track record. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Figure 6. 96/384-well plate formats. M5. San Jose, Calif. RUO. B. Round well design with a clear flat bottom 2. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. 2. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. x. Service Contracts, Preventative Maintenance and. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. Browse for different fluorescent readers below and compare across different instrument suppliers. 384-well TC plate, Poly-d-Lysine, Flat. It has a. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. X. Bioz Stars score: 86/100, based on 1 PubMed. Source plate capacity; 1 x plates. Reader SpectraMax® Paradigm® Multi-Mode Microplate Reader. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. 8 mm distance between well bottoms to plate bottom. For more sample throughput, both readers can be easily. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Efficiency. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535 (Molecular Devices P/N 5089097). x. SpectraMax ID5 Plate Reader (Molecular Devices). Extended linear dynamic range. Polypropylene deep well plates: Elevated well walls for easier sealing Round. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Select Yes at the warning. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. molecular interaction studies, and nucleic acid quantification, as well. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. Supersedes 1. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Microplate Readers. The plate was also read at z height 1 mm with varying delay and integration times for the donor and acceptor reads. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Shaking: Linear and Orbital. Microplate readers are commonly used in life science laboratories. 2. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. Ensure your assay quality with quality tips Certified RNase-/DNase-free Nonpyrogenic Nonsterile 50 racks/case. 340 PC384. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The VICTOR Nivo. Temperature Control: ambient from +4 to 45C. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 7°C (gradient heating design). These advertising cookies will no longer be set for this browser and device. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. 6-384 (96- and 384-well with pipetting) 96-well microplates. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Instrument settings are listed in Table 1. 2, prime the machine with CellTiter-Glo ® 2. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. x. Visit the supplier page or submit a quote to learn more. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Optional. Industry standard disposable (choose from recommended list). Expanded wavelength coverageIn the software select Tools > Configuration. M2. 20 attomol ATP per well. Outer wells aligned for proper viewing with FLIPR Ideal. the Molecular Devices SPECTRAmax® 190 and 250 plate. Biotek Synergy 2 Plate Reader Multi-Mode. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. x. 00. 012 fmol 0. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. 9. Values are averages from 5 clean plates, each filled with 200 µL water/well. ZERO BIAS - scores, article reviews, protocol conditions and more. All stand-alone instrument functions can be controlled using the software. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices'. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes <2 fg/well for firefly luciferase in 96- and 384- well top read. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. 384-Well Microarray/High Sample Recovery Plate. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. 1 a. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. 2. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Plate Formats: 6- to 384-well plates. It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. VIS absorbance detection. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. Embla washers are durable and easy to maintain. Ensure data accuracy of your reader. 1 pM 1536-well: 0. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Selects either 96-well or 384-well plate for a stand-alone read. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Bioz Stars score: 86/100, based on 9 PubMed citations. Overview. • The FlexStation™ II instrument is designed specifically for 96-well microplates. Cells toxicity was corrected for the culture medium background absorbance. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. We would like to show you a description here but the site won’t allow us. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. 190. Integrated stacker option. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. Visit the supplier page or submit a quote to learn more. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Outer wells aligned for proper viewing with FLIPR. 48 ± 0. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Kinetic assays were read every 20-28 seconds for one hour,measurements in compatible Molecular Devices® microplate readers. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. 100 fmol 384-well (75 µL) 0. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. CO 2 & O 2 control. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. x. We also prefer that fluorescence signals in one well don’t bleed into the other, as this means less background and more precise data. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Select Consumables. If you have an xml plate object, then. Two different agonists, acetylcholine and carbachol, were tested. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. Scan every well . The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. 0. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. x. 3 µl). VIS absorbance detection. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 384-WELL SBS STERILE MICROPLATE. Ideal for miniaturized 384 well fluorescent. Synergy NEO multi-mode plate reader: BioTek: n/a: EL406 plate washer and bulk dispenser with wide-bore tip 10 μL cassette: BioTek: n/a:. $913. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. Fluorometers also vary in the size of samples they accept, with some using very small sample sizes to conserve precious sample materials (down to 0. Access this by going to. This is equivalent to having 511 built-in filters, 384-well: 0. 96-Deep Round or Square Well Plates. For Raman excitation, a continuous-wave (CW) laser at 532 nm (Verdi V-18, Coherent) was used. 96-1536. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. A snapshot of the Settings screen is shown in Figure 2. The. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. flat-bottom: 50-60 µl. #3635) Transferpette micropipette (Drummond Scientific cat. Get Quote . smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. The fluid surface in a 384-well is a deeply curved meniscus. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Features. Instrument pricing. Analyst, M5, Flex 3, and SpectraMax L readers work differently. 96-, 384-, and 1536-well microplates. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Description. Molecular Devices, LLC. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. Download PDF. a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. Comprehensive solution. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. It comes standard with 4 wash/rinse bottles and 1 waste bottle for maximum washing flexibility. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). flat meniscus), but is approx 15 mL. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. Molecular Devices has provided scientists with tools to expand the boundaries of their research. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Unparalleled performance on a personalized platform. Molecular Devices 大中华区 Email: info. • 11/10 • Printed in USA • PN: 0120-1525. PURPOSE. 3% in white 96- and 384-well microplates Time. 50 μL total well volume, with recommended working volume of 5-40 μL. 190. The equation that allows one to calculate absorbance from % transmittance is. In the M2, M5 and Plus. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Supplier: Molecular Devices. 7. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 96-well fluorescent cell dilution series, top-read. 100 fmol luciferase per well. Temperature control: 4°C above ambient to 45°C. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. A = Log 10 (I 0 /I). the use of a reader calibrated with primary NIST and NMI standards. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. B. NanoQuant plate. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. We would like to show you a description here but the site won’t allow us. 305 reference level saturation. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1.