Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. S. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. Bacterial growth was determined turbimetrically using the microplate ELISA reader VERSAmax (Molecular Devices). (VersaMax Molecular Devices, Sunnyvale, CA). Turn the reader on and secure the USB cable. We would like to show you a description here but the site won’t allow us. Tristar 3 Multimode Microplate Reader. Measurement of brain-derived neurotrophic factor (BDNF) release. HepG2 cells were seeded into 6-well plates. 10. 7. Select the Driver tab. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. S. 00. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. 0A Sunnyvale, CA Made in USAVersaMax price. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. SoftMax® Pro Software Validation Package provides the most comprehensive documentation and tools available to validate GxP administrator features, software. View the supported Window operating systems for the different versions of the SoftMax Pro software. The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). The percentage of cytotoxicity was calculated using the following formula: cytotoxicity (%) = [1 − (At/As) × 100], where At and As refer to the absorbance of a tested. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Our mission: find your user guide or user's manual as quickly as possible. Ann Arbor, MI, USA). Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). Molecular Devices also offers a mass spectrometry-based solution for food safety testingWe would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. SpectraMax®, VersaMax, VMax. After 72-h of incubation, CellTiter 96 Aqueous One Solution was added and cells were incubated for a further 2 h. +44-118-944-8000. After freeze-drying, the. 4. Our absorbance plate readers feature our PathCheck Sensor technology and industry-leading SoftMax® Pro Data Acquisition and Analysis Software. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. E. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. 00 Regular price. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. absorbance readers. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. (Seongnam, Korea). +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan Nihon Molecular Devices Osaka Tel. VERSAmaxTM Tunable Microplate Reader Operator s Manual for Instruments with Serial Numbers Beginning. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. ). Used. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. Lihat Video. Whether bacteria were partially or completely inactivated by bone cement. The absorbance was assumed to be 100% for the control group. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 003 OD ±1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. All animal experiments were performed in compliance with the relevant laws and. We would like to show you a description here but the site won’t allow us. 4. The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. A. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The. 5 mM. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. All. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The value for non-specific absorption at 600 nm was subtracted. The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. 9000-0756. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. MiR-21. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. 8 in. 2. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high-qualityspectrophotometer. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. Monday to Friday 7am - 5pm Pacific Time. Proliferation was assessed by bromodeoxyuridine (BrdU) incorporation (Cell Signaling Technologies). 00 Sale price $ 0. Substrates and coenzymes provided and specified in the kit were added at 37°C after 1 min plate was read in a spectrophotometer (Versamax, Molecular Devices, San Jose, CA, USA) at 37°C every 1 min for 4 min. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. Preparation of cytoplasmic and nuclear protein. All analyses were performed three times, and the mean values were used for further calculations. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. These values were transformed to logarithmic scale to create a log-log curve. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing. , Sunnyvale, USA). 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. The absorbance was measured at 570 nm and. Cyanobacterial strains that did not lyse until the 12th day were defined as insusceptible. 00: Description. Sponsored. Published Dec 16, 2020 | Updated May 19, 2023. After treatment with GA3The absorbance was measured with a microplate reader (VERSAmax, Molecular devices, San Jose, CA, USA) at 570 nm according to the manufacturer’s instructions. We would like to show you a description here but the site won’t allow us. If the computer is connected to the Internet: Select Search Automatically for. Absorbance plate reader (e. The well plates were then washed with 1% acetic acid and dried. Louis, USA) was used to. 0, 0. Results are presented as the geometric mean and are derived from at least three experiments. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. com ©2010 molecular devices, inc. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. 17. 0 N sulfuric acid. Select up to two wavelengths. 23,225, Thermo Fisher Scientific). S. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. 8). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. used. Four wash/rinse bottles are included and configurable for both 96- and 384. With a wavelength range from 340 to 850 nm, it is the equivalent to having 510 free filters. , Sunnyvale, CA). +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. 0 nm full width half maximum. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. (JC-1) (Molecular Probes), respectively. Statistical Analysis. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. Indocyanine Green Depuration Assay. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. After 30-min incubation, the absorbance was measured at 405 nm wavelength using a spectrophotometer device (VersaMax Molecular Devices, Sunnyvale, CA, USA). Access all the resources, tools and information you need to stay productive. To make your request, nothing more simple: you just have to connect on the site and to make your request on line. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. All experiments were repeated three times. 9. Approximately. 5°C @ 37 °C W. A. 0%, 0-2. Supplier: Molecular Devices. $495. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both. Select Add or Remove Programs system settings. The water extract afforded the greatest yield, and total phenolic and tannin. 3 Methods 1. Open the Windows Task Manager, go to the Services tab, and click Open Services. Click SoftMax Pro Software User Guide. Find Fanuc, Molecular Devices, Eagle, and GE for sale on Machinio. We would like to show you a description here but the site won’t allow us. This reliable model allows for. +86-10-6410-8669 fax +86-10-6410-8601 Molecular Devices Shanghai Tel. Bovine serum albumin was used as a reference. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. p-nitrophenol (Sigma) with diluted concentrations ranging from 0 to 0. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. VersaMax ELISA Microplate Reader. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. Windows 10 – 2004/20H2/21H1/21H2. VersaMax™ ELISA microplate reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 to 850 nm). g. ¨ We would like to show you a description here but the site won’t allow us. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. . The absorbance was read at 570 nm using a spectrophotometer (VersaMax, Molecular Devices, San Jose, CA, USA). Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. 3860 N First Street San Jose,CA 95134 USA +1 408. ImmunohistochemistryAfter 1 h incubation at 37 °C, the color intensity was measured at 562 nm by a microplate reader (model VERSAmax; Molecular Devices, San Jose, CA, USA). It features a modular design that allows users to add or change detection cartridges according to their needs. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. After waiting 15 minutes for colour development at room temperature, the reaction was stopped with 1 M H 2 SO 4, and optical density values were read using an ELISA reader (Versamax, Molecular Devices, LLC, USA) at a wavelength of 492 nm, and the results were given as OD values. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. The. 1700 +1 800. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 2 and 648. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. $1,195. A standard curve was made using the known concentrations of 4-nitrophenol (Sigma). The cortisol-driven molecular programming of HPI axis development has focused only on GR signalling in fishes 12,13,14,15,16,17. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. The cellular viability. Wavelength Bandwidth °¤ 2. Measurement of biofilm formation inhibitory activity. MOLECULAR DEVICES VMAX KINETIC MICROPLATE READER. 파장 범위(nm) : 340–850. Each sample has a discrete sample beam and reference beam so that each well is measured directly. VersaMax™ Tunable Microplate Reader, Molecular Devices. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. 3. #VERSAMAX) SoftMax Pro GxP Software version 5. 0%, 0-2. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). Measurement of changes in BDNF production and release was done byMicroplate Readers. $199. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. While the cell covers about. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. 4. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. ces at one of the following telephone numbers: 408 747-1700 800 635-5577 U. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). (Molecular Research Center, USA) according to the manufacturer’s. Cell survival rate of each sample was calculated as a percentage relative to cells incubated in D-MEM. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 2. The sections were stained with hematoxylin and eosin (H&E) and. The growth inhibitory rate (GIR) of treated cells was calculated by Equation 1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The narrowly focused light beam and optical design give you the same high performance with round bottom, flat bottom, or half area well plates. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. SpectraTest ABS1 Absorbance Validation Plate. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. The signal was read on an ELISA plate reader (SpectraMax VersaMax, Molecular Devices, Sunnyvale, CA, USA). By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Then the medium was removed and 200 μL of DMSO was added to each well to solubilize the formazan crystals. Cell viability was quantified by microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA) at 570 nm. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. 747. 4. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. +06-6399-8211 Fax +06-6399-8212 ©2002 Molecular Devices Corporation. Molecular Devices Beijing Tel. 0. from Molecular Devices Corporation. # SMP54-GXP- 10UL) Pyrochrome Assay. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. This manual is subject to change without notice. 00 Regular price. Concentration was determined by BCA Protein Assay Kit (ref. and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. com we know that an user's manual may be needed at any time. If you are looking for a comprehensive user guide for SoftMax Pro 7. VERSAMAX. To update the driver. Get pricing and purchase lab equipment and instrumentation today. Used. Sale Sold out. Table 1. 7. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY $3,450. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Spectrophotometers. 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. We would like to show you a description here but the site won’t allow us. No accessories are needed and SoftMax® Pro Software. In general terms, plasma was added to a 96-well plate containing the targeted antisera, after rigorous washing steps and conjugation with a secondary detection antisera, and the resultant colorimetric reaction was read by scanning spectrophotometry (VersaMax, Molecular Devices, Sunnyvale, CA). The SpectraTest® ABS1 Absorbance Validation. reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. StakMax Microplate Handling Stacker. SpectraTest® Validation Package. 72 MB. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. The sections were stained with hematoxylin and eosin (H&E) and. 키네틱 읽기 시간 : 최소 9초 간격. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. 90 shipping. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. After incubation, the absorbance of the plate was measured at 450 nm using a microplate reader (VERSAmax™, Molecular Devices Corp. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. 2. 00. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 99 + $42. Cells were lysed in NETN buffer (100 mM NaCl, 20 mM Tris pH 8. Reverse transcription-quantitative polymerase chain reaction To assess phospholipase C gamma 1 (PLCG1) and phospholi-pase C gamma 2 (PLCG2) mRNA levels in each. The reaction was stopped with 100 µL of H 2 SO 4 1 M and quantified in a spectrophotometer (VERSAmax, Molecular Devices, San Jose, CA, USA) at 450 nm. The index was evaluated at 48 and 72 hours normalized to that at 24 hours. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. SpectraMax®, VersaMax, VMax. ®ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. And the VERSAmax reader is robotics-compatible. This document is provided to customers who have purchased Molecular Devices, Inc. This manual is subject to change without notice. Absolute values were attained with a standard curve composed of type I bovine skin collagen solution (0. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). $1,195. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. New and used Molecular Devices VersaMax's for sale on LabX. TECHNICAL NOTE. We would like to show you a description here but the site won’t allow us. For more sample throughput, the system can be easily integrated into a full roboticThe VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. And the VERSAmax reader is robotics-compatible. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Thus, the molecular mechanism promoting hepatic lipid accumulation in obesity is of biomedical and public health interest. StakMax Microplate Handling System. 4. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Used. 5 mg/mL) in the range of 5–100 μg per 0. Windows Home Editions are not supported. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Monday to Friday 8am - 5pm GMT. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Select Start>Control panel>Device Manager. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). Cell viability was expressed as percent survival of untreated cells. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. Finally, the absorbance at 450 nm was detected by an ELISA reader, VERSAmax (Molecular Devices, San Jose, CA, USA). Reboot the computer. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence.