The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. RIDASCREEN Gliadin competitive ELISA. Multimode Reader Comparison Chart. 0´ 104 6. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax i3x Register or Sign In. The. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. User-upgradeable detection modules allow you to expand the capabilities of your microp. 蛍光 発光 ELISA. It allows you to explore cellular . SpectraMax iD3 and iD5 Register or Sign In. 1. techsupport. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. SpectraMax® i3x. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. . Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. eu@moldev. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. GFP expression imaged on the cytometer. Shake intensity. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. VAL014, right table) assays on the SpectraMax i3x reader are shown below. 0´ 104 1. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . . This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The spectra analysis and K d were determined using Sigmaplot 14. . Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Find regional support. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Structured Review. Published Dec 16, 2020 | Updated Apr 13, 2023. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. viii SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 340PC control panel are shown in boxed Helvetica type. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. The SoftMax® Touch Software is installed in. 9 x 10-15. 100 fmol 384-well (75 µL) 0. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 3) At the bottom of the strip, click on "Show. The chamber. Register to download your free eBook today. 3: The cuvette chamber. Danaher Inc spectramax i3x. 7 Figures from 1126 Publications Promega. Example: [Drawer]. Facilities. Assay setup for a 384-well low-volume plate. 020 fmol 0. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. Overview of Western Blot Cartridge. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. i3x single use: $35: $52. ü; ü. • Removing the transport locks from the PMT shutters and drawers. EUROPE. Wokingham, Berkshire RG41 5TS United Kingdom. . Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Figure 4. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Videos & Webinars. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Find regional support. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. Description. 5 Figures from 202 Publications Thermo Fisher Scientific. 2 nM was calculated using SoftMax Pro Software. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Bioz Stars score: 98/100, based on 1 PubMed citations. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. SoftMax Pro User’s Manual v Contents Figures. . Replicate values were averaged and imported into Design-Expert 8. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. As. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax M5. Products : SoftMax Pro GxP Software, SoftMax Pro Software. . 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. Supplier: Molecular Devices. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Serial number 33 900 01-3387. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). . . Figure 1. The protocols are also available on the SoftMax Pro Software protocol sharing. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. SpectraMax i3x, onboard luminescence 121. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 00800 665 32860. Supplier: Molecular Devices. HTRF-compatible SpectraMax Multi-Mode Readers. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. . Biochrom New EX Read 400 Microplate Reader. Prepare your i3/i3x instrument for shipment. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. HeLa cells were plated at 15,000 cells per well in 100 µL. STAKMAX. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Quantiferon-TB Gold in Tube Kit. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. . 0´ 104 8. 99. The plate was shaken and incubated in the dark for 10 minutes. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . china@moldev. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Simplified workflow: Add cellular imaging assays in a small footprint. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Find our technical support information along with contact details. If no sound is heard during initialization, contact technical support. SpectraMax® i3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 4. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. \r Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48 AM The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Align the two slots on the bottle holder with the two pegs on the rail. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 12911151) 0. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. 302 low ref light. The corrected absorbance is within 5% of the. Bulk Kit. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. 7. Remove the transport bracket and store the retaining screws in the bag that held the 3. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. user upgradeable. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Bioz Stars score: 98/100, based on 1 PubMed citations. Microplate optimization should be performed the first time the assay is run, and subsequently for . The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. . Nucleic Acid and Protein Quantitation. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 1 Microplate Data Acquisition & Analysis Software for Windows. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. . Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. . SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Controls containing all reaction. Determination of anti-inflammatory. The dynamic range of the assay is about 3. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. SoftMax Pro. EMax Plus Microplate Reader. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 7 mm for all shake intensities. g. Find regional support. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. 99 to $1999. com. Settings not required by a reader are indicated by ‘---’. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. . 660-665 Eskdale Road. The CellTiter-Glo assay from Promega uses luciferase. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Figure 2-23: Bottle holder To install the bottle holder: 1. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. . After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. o For example: Ex: 485/Em 525/Cutoff 515 4. Table 1. 0. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. The information below applies to a standard 96 well plate, set to a linear shake mode. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Italic and boldface type are used for emphasis. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. 더 많은. applications, including dual luciferase and ATP assays. Explore a wealth of applications with the future-ready SpectraMax i3x. The calculated LOQ values were 0. The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. Binding and Inhibition. to View Figures. Average 98 stars, based on 1 article reviews. 303 unable to cal dark current. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectramax I3x, supplied by Danaher Inc, used in various techniques. . SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The dual auto injector module. Molecular Devices ScanLater Western Blot Detection Platform. Not compatible with Home Edition. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. We would like to show you a description here but the site won’t allow us. 1. GloMax Discover System. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 吸収度マイクロプレートリーダー SpectraMax® Mini. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. Description 1. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. SpectraMax i3x Multi-Mode Microplate Reader. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. 1. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. Learn more about its features, specifications, and applications in this user guide. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Background Parallax,Columns 2. Antigen Quantitation Protocol. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). Spectranet customer portal. 9000-0756. . ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Examples: “Press carefully to engage,” “ Do not press down. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. ImageXpress Confocal HT. ; The top 3 importers of Spectramax are India with 365. This process should take about 3 - 5 minutes. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. i3x Specifications. Bioz Stars score: 98/100, based on 1 PubMed citations. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 2 x 10-14. R8362. SpectraMax i3x readers. Unlimited breadth of. ZERO BIAS - scores, article reviews, protocol conditions and more. StackMax Microplate Handling System. . Failed to load PDF file. Supported Windows Operating System: See release notes. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. HTRF IP standard curve. . SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Overview of Western Blot Cartridge. Monday to Friday 8am - 5pm GMT. Calcium. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. *On the SpectraMax i3x reader only. . SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. SpectraMax i3x and Paradigm readers require the FP-FLUO. GloMax ® Navigator System. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. The plate was covered and incubated for one hour at room temperature. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. 1. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 1 software Download page. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). 01 0. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. Tristar 5 Multimode Microplate Reader. each new assay. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 315 can't find zero order. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Read modes. SpectraMax i3, i3x, and iD5 readers. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. . SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Whether you are looking for an intuitive, easy-to-use reader for. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. . The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Description 1. Winnersh Triangle. 1 and above: Compatible with only with 64-bit OS. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . . DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). +44-118-944-8000. . SeeSpectraMax MiniMaxImagingCytometeronpage85. Manufacturers try to design their instruments so they can be. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. Prepare your iD series instrument for shipment. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Nuclear Receptor. Data Analysis. No accessories are needed and SoftMax® Pro. A: percent GFP-positive cells calculated using the classification feature in the software. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change.